Journal: Frontiers in Cellular Neuroscience

Article Title: Oridonin attenuates TLR4-driven inflammation and autophagy in LPS-stimulated enteric glial cells: an in vitro and in silico analysis

doi: 10.3389/fncel.2026.1748505

Figure Lengend Snippet: Effects of LPS and oridonin on caspase-1 and IL-1β levels in enteric glial cells. Data represent mean ± SEM from three independent experiments, each performed in technical replicates. Statistical analyses were performed using one-way ANOVA followed by Tukey’s post hoc test. * p < 0.05, ** p < 0.01, **** p < 0.0001. LPS: Lipopolysaccharide; O1: 1 μM oridonin; O2: 5 μM oridonin.

Article Snippet: Commercially available ELISA kits for rat caspase-1 (BT Lab, E1897Ra) and IL-1β (BT Lab, E0119Ra) were used according to the manufacturer’s instructions.

Techniques:

Journal: Experimental and Therapeutic Medicine

Article Title: 2,3,5,4′-tetrahydroxy-stilbene-2-O-β-D-glucoside attenuates methionine and choline-deficient diet-induced non-alcoholic fatty liver disease

doi: 10.3892/etm.2018.6300

Figure Lengend Snippet: The effects of TSG on protein expression of NLRP3, ASC and caspase-1 in livers of MCD diet fed mice. Protein imprinting of NLRP3, ASC, and caspase-1. (A) As a matter of fact, nine samples were included in the original gel/experiment. The antepenultimate and penultimate pair were omitted from the figure (as denoted by the dotted lines) since they were not relevant for a discussion of the experiment at hand. Densitometric analysis of (B) NLRP3, (C) ASC, (D) caspase-1. Mice fed an MCD diet showed increased levels of NLRP3, ASC and caspase-1 protein expressions compared with mice in the control diet-fed group. The results showed that the middle dosage of TSG downregulated ASC and caspase-1 levels. ## P<0.01; ### P<0.001 ( # , compared with MCD diet-induced NAFLD model group). *P<0.05; **P<0.01; ***P<0.001 (*, compared with the control group). TSG, 2,3,5,4′-tetrahydroxy-stilbene-2-O-β-D-glucoside; NLRP3, Nod-like receptor protein 3; ASC, apoptosis-associated speck-like protein containing a C-terminal caspase recruitment domain; MCD, methionine and choline-deficient; NAFLD, Non-alcoholic fatty liver disease.

Article Snippet: Antibodies directed against NLRP3, apoptosis-associated speck-like protein containing a C-terminal caspase recruitment domain (ASC) and caspase-1 were purchased from Proteintech Group, Inc. (Chicago, IL, USA).

Techniques: Expressing

Journal: Journal of Nanobiotechnology

Article Title: Manganese-enriched nanoboron agent amplifies BNCT efficacy via pyroptosis-mediated immune activation and STING pathway synergy​

doi: 10.1186/s12951-025-03916-8

Figure Lengend Snippet: Pyroptosis Induced by BSA- 10 BPA-MnO 2 after BNCT. ( a ) Fluorescent images of ROS in B16F10 cells after different treatments, detected by DCFH-DA staining. Scale bar: 150 μm. ( b ) Cell morphology images after different treatments. In the BSA- 10 BPA-MnO 2 + N group, cell swelling and the appearance of large bubbles (blue arrows, one of the most prominent features of pyroptosis) are observed. Scale bar: 50 μm. ( c ) Western blot analysis of caspase-1 (CAS-1) and cleaved caspase-1 (cleaved-CAS-1). ( d ) Immunofluorescent staining of the GSDMD protein N-terminal fragment (GSDMD-N). Scale bar: 100 μm. ( e ) Relative LDH release, n = 4. Data are presented as mean ± SD. * p < 0.5, **** p < 0.0001

Article Snippet: After blocking, membranes were probed with primary antibodies against caspase-1 and cleaved caspase-1 (MedChemExpress, China), followed by HRP-conjugated secondary antibodies.

Techniques: Staining, Western Blot

Journal: Experimental Biology and Medicine

Article Title: Mechanisms of AGE-induced VSMC phenotypic switching and macrophage modulation in human abdominal aortic aneurysms

doi: 10.3389/ebm.2025.10527

Figure Lengend Snippet: AGEs Activate the NF-κB Pathway and NLRP3 Inflammasome via the RAGE/RhoA/ROCK Pathway. (A) The TransAM NF-κB Transcription Factor Assay detected the effect of the RAGE/RhoA/ROCK inhibitor on the nuclear translocation of c-Rel, p52, and p65; (B) The NF-κB dual luciferase reporter gene system assessed the impact of RAGE/RhoA/ROCK inhibitors on AGE-induced NF-κB signaling; (C) Western blot analysis showed that AGEs regulate the expression of NLRP3 via the RAGE/RhoA/ROCK/NF-κB signaling pathway; (D) The effects of the inhibitors on caspase-1 activation were assessed using a caspase-1 assay kit; (E) An LDH release assay showed that AGE promoted VSMC cell pyroptosis, but it could be reversed by RAGE, RhoA, and ROCK inhibitors; (F) An ELISA assay demonstrated that AGE promoted the release of the inflammatory factor IL-1β through RAGE/RhoA/ROCK. Data are presented as the mean ± SD from three independent experiments and were analyzed with a one-way ANOVA followed by a Dunnett’s multiple comparisons test. Asterisks indicate significant differences (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001) between the treatment group and the control group. Pound signs indicate a significant difference (#P < 0.05, ##P < 0.01, ###P < 0.001) between the treatment group and the AGE group.

Article Snippet: The enzymatic activity of caspase-1 was assayed using a Caspase-1 Colorimetric Assay Kit (MCE, Cat. No. HY-K2610-100T) according to the manufacturer’s protocol.

Techniques: Transcription Factor Assay, Translocation Assay, Luciferase, Western Blot, Expressing, Activation Assay, Lactate Dehydrogenase Assay, Enzyme-linked Immunosorbent Assay, Control